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. 2005 Dec 15;25(1):255–265. doi: 10.1038/sj.emboj.7600910

Figure 4.

Figure 4

Gene silencing of Adi3 causes localized cell death. (A) Adi3 silencing causes cell death in leaves and stems. Cell death lesions were photographed 3 weeks after TRV:Adi3 infection. Arrows point to localized regions of cell death. In the left panel, leaves were cleared in 10% acetic acid, 30% chloroform, 60% EtOH. (B) Adi3 silencing causes stunting. (C) Adi3 transcripts are reduced by silencing. Control (TRV) and Adi3-silenced (TRV:Adi3) tissues were analyzed by RT–PCR for Adi3 transcripts. Aliquots were removed at the indicated cycles. Actin was the control. NC, negative control. (D) Adi3 silencing does not affect transcript levels of closely related AGC kinase genes. Tomato plants were silenced for the indicated genes (across top) and analyzed for transcript levels by RT–PCR (right side). EF1α was the control. Lanes show products from cycles 28, 34, and 40 and MW markers.