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. 2005 Dec 15;25(1):255–265. doi: 10.1038/sj.emboj.7600910

Figure 5.

Figure 5

Pdk1 and Adi3 negatively regulate plant cell death. (A) OSU-03012 inhibition of tomato Pdk1. Top, dose-dependent inhibition of recombinant Pdk1-FLAG autophosphorylation. Bottom, α-FLAG Western blot showing assay input. (B) Pdk1 inhibitor OSU-03012 induces cell death in plants. Tomato protoplasts were treated with the Pdk1 inhibitor OSU-03012 and cell viability was monitored over 24 h. (C) Attenuation of OSU-03012-induced cell death by overexpression of Pdk1, Adi3, or Adi3S539D. Tomato protoplasts overexpressing proteins for 9 h were treated with OSU-03012 for an additional 12 h and cell viability was determined. The right panel shows Western blot detection of overexpressed proteins. (D) Lack of attenuation of OSU-03012-induced cell death by overexpression of kinase-deficient Pdk1K77Q or Adi3K337Q. The right panel shows Western blot detection of overexpressed proteins. (E) Agrobacterium-mediated overexpression of Adi3 can suppress cell death induced by PtoY207D in leaf tissue. The lower panel shows Western blot detection of overexpressed proteins; HA-Adi3, filled triangle; Pto-HA, open triangle.