FIG. 1.

Schematic representation of mutations in the PCNA-binding motif of Apn2 and in PCNA. (A) Mutations in Apn2. The N terminus of Apn2, which contains the conserved sequence motifs characteristic of this family, is shown as a white box. In the C terminus, the sequence of the PCNA-binding motif is shown as a black box, and the conserved amino acids are shown above. The asterisks indicate the amino acid residues that were changed to alanines in the Apn2 A⁴³⁰A⁴³³A⁴³⁴ protein. The gray box represents the sequence that bears homology to the tandem repeat of topoisomerase III. (B) Mutations in PCNA. The sequences of the IDCL and the C-terminal tail of PCNA are shown. The amino acid residues that were changed to alanines in the IDCL region in pcna-79 and in the C-terminal region in pcna-90 are indicated by asterisks.