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. 2002 Sep;22(18):6480–6486. doi: 10.1128/MCB.22.18.6480-6486.2002

FIG. 2.

FIG. 2.

Physical interaction of Apn2 with PCNA by gel filtration. In the reactions, 3.5 μg of the Apn2 protein and 5 μg of PCNA were incubated in different combinations and gel filtered. (A) Wild-type Apn2 with PCNA. (B) Mutant Apn2 A430A433A434 with PCNA. (C) Wild-type Apn2 with pcna-90. (D) Wild-type Apn2 with pcna-79. The fraction numbers and the elution positions of the molecular mass markers for the gel filtration column are shown on the top. The positions of molecular mass standards on the protein gels are indicated on the left.