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. 2002 Sep;22(18):6498–6508. doi: 10.1128/MCB.22.18.6498-6508.2002

FIG. 3.

FIG. 3.

SENP2 localizes to the nucleoplasmic face of the NPC. (A) Cells were fixed, permeabilized with either acetone (panels a and c) or digitonin (panels b and d), and probed simultaneously with a rabbit anti-myc antibody and a mouse monoclonal antibody specific for RanGAP1. RanGAP1 (panels c and d) localizes to the cytoplasm and to the filaments on the cytoplasmic face of the NPC (19) and is detectable in cells permeabilized with both acetone and digitonin. SENP2 (panels a and b) localizes to the nucleoplasmic face of the NPC and is detectable only in cells permeabilized with acetone. (B) Cells were fixed, permeabilized with either acetone (panels a and c) or digitonin (panels b and d), and probed simultaneously with a mouse anti-myc monoclonal antibody and a rabbit antibody specific for lamin B. SENP2 (panels e and f) and lamin B (panels g and h) are detectable only in cells permeabilized with acetone. Bar, 10 μm.