FIG. 2.
Effects of E2a-Hlf compared with p190Bcr-Abl on the in vitro growth of primary bone marrow progenitors. (A) Typical colonies arising on the underlying stromal layer in primary bone marrow cultures 2 weeks after initiation with cells transduced by MSCV-E2A-HLF (left) and MSCV-BiEH (right). Clusters of viable and apopotic cells are indicated by black and white arrows, respectively. (B) Effects of BCR-ABL and E2A-HLF ± BCL2 on the in vitro growth of hematopoietic cells. Each point represents the fold increase in number (mean of triplicate determinations) of cells adherent to the underlying AC-6.21 stromal layer.