FIG. 7.

p53 pathway alterations correlate with stroma-independent growth of B-cell progenitors immmortalized by E2a-Hlf. (A) Western blot analysis of total cellular proteins isolated from cells growing in bone marrow cultures (identities indicated above gel lanes). Antibodies are indicated to the right of gel lanes. (B) Western blot analysis was performed with a sheep anti-p53 serum on immune complexes resulting from immunoprecipitation with a monoclonal antibody that recognizes an epitope exposed by conformational changes associated with a subset of p53 missense mutations (6).