FIG. 2.

dPDZ-GEF is required for normal eye development. Flies of the following genotype were examined by SEM and tangential section: w¹¹¹⁸ (A and B); dPDZ-GEF¹/dPDZ-GEF² (C, D, and E); dPDZ-GEF^rv-1/dPDZ-GEF^rv-1 (F and G); y w ey-flp/y w; dPDZ-GEF¹ FRT40A/FRT40A (H and I). Shown in panel E and in the insert in panel D are flies of the same genotype as those shown in panel C and in the upper portion of panel D, grown at 30°C for a more severe phenotype. The dPDZ-GEF^rv-1 allele (F and G) was obtained by precise excision of dPDZ-GEF¹ allele. Shown in panel J is the same eye as that in panel H, photographed by light microscopy. Arrows in panels H and J indicate the same area containing dPDZ-GEF¹ homozygotic clones. The red pigment indicates the existence of the P-element, which causes a loss of function of dPDZ-GEF in that area. The dotted line in panel I indicates the boundary between mutant (−/−, upper left part) and normal (+/+, lower right part) ommatidia.