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. 2006 Jan 30;34(2):709–720. doi: 10.1093/nar/gkj464

Figure 2.

Figure 2

Effects of amino acid substitutions in the Hfq protein on RNA binding and stimulation of poly(A)polymerase elongation. (A) Relative interaction of wild-type and mutant Hfq proteins with polyadenylated 3′-rpsO RNA fragment assayed by gel retardation. Radiolabelled 3′-rpsO-(A)18 RNA (10 pM) was incubated without (lane 1) or with 50 pM (lanes 2, 4, 6, 8, 10, 12 and 14) and 500 pM (lanes 3, 5, 7, 9, 11, 13 and 15) of either wild-type Hfq protein (wt) or of the mutant protein indicated at the top. (B) Poly(A) polymerase activation by wild-type and Hfq variant proteins was assayed on the 3′-rpsO RNA fragment. Radiolabelled RNA fragment was incubated with the Hfq protein indicated at the top of the gel at the concentration specified, in the presence of tRNA and ATP. PAP was added at time 0. Samples were removed and reaction stopped at the times indicated at the bottom of the picture. c indicates RNA incubated without PAP.