Figure 3.
Effects of overexpression of Hfq variants on cell growth. Growth curves of N3433 (hfq+) and IBPC 953 (hfqΔ) strains transformed with pACYC184 derivative (upper part) and pCL1921 derivative (lower part) plasmids containing hfqwt (giving pCLhfq+ and pTX381), hfqV43C (giving pCLhfqV43C and pTX381hfqV43C) and hfqV43R (giving pCLhfqV43R and pTX381hfqV43R), respectively. Cells were grown in LB media with appropiate antibiotics at 37°C. Turbidity of the cultures was monitored at 650 nm.