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. 2006 Jan 30;34(2):709–720. doi: 10.1093/nar/gkj464

Figure 4.

Figure 4

Complementation of the hfq10::cat mutation with Hfq variants assessed by expression of the rpoS-lacZ chromosomal-integrated fusion. MCM11 is a hfq10::cat derivative of RO91 carrying a chromosomal rpoS-lacZ fusion. It was transformed with pCL1921 derived plasmid expressing either wild-type or hfq gene variants (V43C and V43R) giving the pCLhfq+, pCLhfqV43C and pCLhfqV43R plasmids, respectively. The empty vector was used as a control (pCL1921). β-Galactosidase synthesis from the rpoS–lacZ chromosomal-integrated fusion was measured in cells grown until OD600 of 1.6–1.9 (A) or until OD600 of 0.5–0.6. (B) Each value is the mean of at least three independent experiments, with standard deviations not exceeding 15% of magnitude. (C) Expression of the mutated hfq alleles. Soluble protein extracts from strains grown until OD600 of 1.6–1.9 shown on Figure 4A were analysed by western blotting using Hfq antibodies (upper part of the figure). Lower part of the figure is the quantitation of the western blot data coming from four sets of experiments. Hfq antibodies detected monomers in the extracts.