Table 2.

Effect of hfqV43R mutation on the expression of hfq-lacZ and the Hfq amounts

Strain	β-Galactosidase activity (a)		Hfq protein amount (b)
	(c)	(d)	(c)
hfq+	4150 ± 50	5450 ± 50	39884 ± 4280
hfqV43R	7350 ± 200 (1.7)	7400 ± 200 (1.3)	77638 ± 10716 (1.9)

IBhfq95 and IBhfq95hfqV43R strains carrying a hfq–lacZ translational fusion on the chromosome were grown as described in Figure 3A.

(a) β-Galactosidase levels from the hfq–lacZ fusion. Bacteria were harvested either in exponential phase (c) or upon entry in stationary phase (d). Mean values are calculated from three independent experiments. (b) Relative abundance of Hfq protein in the extracts. Quantification in arbitrary units of the western blot (data not shown), giving the mean values of two independent samples values in parenthesis are the fold increase in β-galactosidase levels or Hfq amount in the hfqV43R mutant compared with the wild-type strain.