Figure 2.
The bud1 Mutant Phenotypes Seem Auxin Related.
(A) Induction of hypocotyl elongation by high temperature. Wild-type and bud1 mutant seedlings were grown on 0.5× Murashige and Skoog (MS) plates at 20 and 29°C for 9 d and photographed with the same magnification.
(B) Comparison of lateral roots between bud1 and wild-type plants, which were grown on 0.5× MS plates for 14 d and photographed.
(C) to (F) Vascular systems of cleared specimens of wild-type ([C] and [E]) and bud1 ([D] and [F]) plants were viewed with dark-field optics. Photographs showing venation patterns of cotyledons ([C] and [D]) and leaves ([E] and [F]) of the seedlings grown on 0.5× MS plates for 12 d were taken with the same magnification.
(G) and (H) Microscopic comparison of the interfascicular fiber differentiation among wild-type (G) and homozygous bud1 (H) stems, showing the presence of three layers of interfascicular fibers in the wild type and one to two layers of sclerified interfascicular cells in homozygous bud1 (arrowhead) or no interfascicular fibers (arrow). Bars = 5 μm.