Figure 5.

In Situ Hybridization Examining the Expression of Cell Cycle Phase–Specific Marker Genes.

(A) CycB1 mRNA expression in wild-type SAM and developing young flowers. The white arrow indicates the SAM. The inset shows a wild-type SAM hybridized with the sense CycB1 RNA probe as a negative control.

(B) CycB1 expression in tso2-1 SAM and young flowers.

(C) H4 expression in a wild-type inflorescence. The black arrow indicates a lack of H4-expressing cells in the stem.

(D) H4 expression in a tso2-1 inflorescence. A dramatic increase in H4-expressing cells in flowers and in the stem (black arrow) is evident.

(E) CycB1 RNA expression in a wild-type 3-week-old seedling.

(F) CycB1 RNA expression in a 3-week-old tso2-1 rnr2a-1 seedling.

(G) H4 expression in a wild-type 3-week-old seedling.

(H) H4 expression in a 3-week-old tso2-1 rnr2a-1 seedling.

Bars = 10 μm.