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Expression of Cell Death Markers in tso2-1 rnr2a-1 Mutants.
Rosette leaves of 10-d-old wild-type and tso2-1 rnr2a-1 plants were examined for PCD using three different histochemical markers: trypan blue staining, H2O2 production, and callose deposition. Dark blue patches stained by trypan blue, reddish-brown deposits (reaction products between 3,3-diaminobenzidine and H2O2), and callose deposition revealed by aniline blue staining indicated PCD in tso2-1 rnr2a-1 mutants.
