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NO Accumulation Localized to Subregions of Chloroplasts in PPIX-Induced Cell Death.
Protoplasts from 18-d-old wild-type leaves were treated with 10 μM PPIX or PPIX + cPTIO (25 μM) for 4 h in the light. Protoplasts were double-stained with DAF-FM (green) and CMXRos (red) and observed by laser scanning confocal microscopy. This experiment was repeated three times with similar results.
