Figure 1.

Gene Organization and Expression of At2g33770 Encoding a Ubiquitin-Conjugating E2 Enzyme.

(A) Exons and five putative miR399 target sites within the second exon are shown as gray boxes and black bars, respectively. The translation initiation site and the ubiquitin-conjugating conserved domain (UBC) are indicated. The triangle indicates the T-DNA insertion site in the SAIL_47_E01 line.

(B) and (C) RNA gel blot analyses of miR399 (B) and E2 (C) transcripts in seedlings grown in high-Pi (+Pi) or low-Pi (−Pi) medium. At4, a Pi starvation–induced gene (Burleigh and Harrison, 1999), was used as a positive control. 5S rRNA and tRNA and 25S and 18S rRNA staining are shown as loading controls. NT, nucleotides.