Figure 4.

Expression of FLS2 in Tomato Cells.

Detergent-solubilized extracts of equal amounts of cells (fresh weight) from tomato, tomato transformed with P_35S:FLS2:3*myc (tomato-P_35S:FLS2), and Arabidopsis were immunoprecipitated with anti-FLS2 antibodies.

(A) Analysis of these immunoprecipitates on protein gel blots with anti-FLS2 antibodies.

(B) Binding activity of these immunoprecipitates tested for binding of ¹²⁵I-Tyr-flg22 without the addition of competitor or with 100 nM flg15 or 10 μM flg22 as indicated. Bars and diamonds indicate means and actual values, respectively, of two replicate measurements.