Figure 2.

RAPTOR1 Interacts with TOR-HEAT Repeats in Vivo.

35S:GST-RAPTOR1 and 35S:GFP-TOR-HEAT repeat constructs were expressed in tobacco leaves (Nicotiana benthamiana) by agroinfiltration. The C-terminal 2-kb fragment of TOR that includes the FRB domain and the kinase domain was fused to GFP and expressed as a control (35S:GFP-TOR-CT). The expressed proteins were immunoprecipitated (IP) using GFP antibody, and the immunodetections of the same fractions were made with GFP antibody (lanes 1 to 3) and GST antibody (lanes 4 to 6). Presence of RAPTOR1 as a coimmunoprecipitant of TOR-HEAT repeats is shown in lane 6, while GST-RAPTOR1 expressed alone (lanes 1 and 4) is not pulled down with GFP-antibody.