Figure 7.

RAPTOR Regulates the Sensitivity of S6K1 to Osmotic Stress.

The 35S:GFP-S6K1, 35S:GST-RAPTOR, and 35S:TOR-HEAT repeat constructs were transiently expressed in tobacco leaves (N. benthamiana) with or without subsequent mannitol treatment as indicated in the figure. Following immunoprecipitation with GFP antibody, the sample fractions were subjected to in vitro kinase assay using GST-RPS6-CT as substrate (top panel), immunodetection with GFP antibody to show the equal loading of S6K1 (middle panel), and another immunodetection with GST antibody to confirm the interaction of RAPTOR with S6K1 (bottom panel).