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Transferrin receptors accumulate abnormally in Rab11-positive endosomes in cells with reduced PLD2 expression. Control and siPLD2-treated cells were labeled with transferrin receptor and Rab11 antibodies (A) or with fluorescent transferrin and EEA1 antibody (B), and confocal micrographs were taken. Most cells in the siPLD2 samples had a prominent perinuclear accumulation of transferrin receptors; typical cells with this phenotype are shown.
