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. 2006 Feb;17(2):623–633. doi: 10.1091/mbc.E05-07-0631

Figure 5.

Figure 5.

Allogeneic CTL attack induces grB-independent DNA fragmentation in ConA-stimulated lymphoblast targets. Two-day ConA-stimulated lymphoblasts (A and B), L1210 (C and D), or 24-h ConA-treated L1210 (E and F) were challenged with alloreactive CTL (5:1 effector/target ratio). ConA-treated targets were washed extensively before CTL attack. DNA fragmentation in targets was monitored by tritium-release at 2 h (A, C, and E), or cell permeabilization by chromium-release at 4 h (B, D, and F). CI-MPR-deficient (G) and CI-MPR-overexpressing (H and I) fibroblast cell lines were challenged with alloreactive CTL, and DNA fragmentation in targets was monitored by tritium release at 3 h (G and H) or 6 h (I). E/T, effector to target ratio; grA, granzyme A; KO, knockout; WT, wild type. Data represent the mean ± SD of three separate experiments (A–F and I); or represent the mean ± SD of triplicate samples, and are representative of at least three separate experiments (G and H; * 0.01 < p < 0.05; ** 0.001 < p < 0.01; *** p < 0.001).