Figure 10.

Depletion of FOP affects MT anchoring. HeLa S3 cells were transfected with GL2 (A) or a FOP-specific siRNA oligonucleotide duplex (B). They were then subjected to MT regrowth assays and fixed at the time points indicated. FOP was visualized with anti-FOP antibodies and MTs were stained with FITC-labeled anti-α-tubulin antibody. Bar, 10 μm. (C) Transfected cells were classified according to their failure to nucleate MTs (white bars), or nucleate MTs and then form asters (gray bars) or nonfocused MT networks (black bars). Histograms show results from three independent experiments, counting 300 cells each, and error bars indicate SDs.