Skip to main content
. 2006 Feb;17(2):634–644. doi: 10.1091/mbc.E05-08-0810

Figure 5.

Figure 5.

CAP350 interacts with FOP in vivo. (A) FOP interacts with a C-terminal fragment of CAP350. Immunoprecipitation experiments were performed on lysates from 293T cells after cotransfection with the indicated plasmids. Antibodies used were anti-myc (lanes 3 and 4) and anti-FLAG (lanes 5 and 6), coupled to beads. Immunoprecipitated proteins were then analyzed by Western blotting, using the indicated antibodies, and whole cell lysates were analyzed in parallel (lanes 1 and 2). In a separate experiment, anti-FLAG immunoprecipitates were also separated by 6% SDS-PAGE and probed with anti-CAP350 antibody to detect endogenous CAP350 (lanes 9-12). (B) Endogenous FOP interacts with endogenous CAP350. HEK293T cell extracts were immunoprecipitated with control rabbit IgG (lane 2) or goat IgG (lane 5) and anti-FOP (lane 3) or anti-CAP350 antibody (lanes 6). Immunoprecipitates were subject to Western blotting with anti-FOP (top) or anti-CAP350 (bottom) antibodies; whole cell lysates are shown in lanes 2 and 4.