Figure 4.
(A) Benomyl sensitivity and epistasis. (A) cdc55Δ mutant in combination with deletions of the checkpoint genes MAD2 and BUB2. Stationary phase cultures were normalized to equal densities (1 × 107 cells/ml) and spotted in a 10-fold serial dilution series on YPD (rich medium) or YPD plus 12 μg/ml benomyl. Plates were incubated at 25°C. Strains were wild-type (8058-4-4), mad2Δ (8426-9-4), bub2Δ (8862-10-2), cdc55Δ (8615-1-4), mad2Δ cdc55Δ (8667-2-3), and bub2Δ cdc55Δ (8818-9-3). (B) Deletion of LTE1 in combination with cdc55Δ. Strains were wild type (8058-4-4), cdc55Δ (8615-1-4), lte1Δ (8505-12-4), and cdc55Δ lte1Δ (8665-17-3). (C) Deletion of the FEAR components SLK19 and SPO12 in combination with cdc55Δ. Strains were wild-type (8058-4-4), cdc55Δ (8615-1-4), spo12Δ slk19Δ (8933-1-3), and cdc55Δ spo12Δ slk19Δ (8933-5-4).