Figure 2.

Cytosolic mislocalization of PpPex8p in cells deficient in both PTS1 and PTS2 import pathways. Wild-type (PPY12) and pex mutant strains expressing both functional GFP-Pex8p and Pex3p-mRFP fusion proteins were grown in synthetic medium (YNM) for 4 h and visualized by fluorescence microscopy and Nomarski optics. Scale bar, 2 μm.