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GFP-Pex8pΔAKL targeting to peroxisomes is dependent on both Pex7p and Pex20p. (A) GFP-Pex8pΔAKL and Pex3p-mRFP were expressed in wild-type and pex mutant strains. Cells grown in synthetic medium (YNM) for 4 h were visualized by fluorescence microscopy and Nomarski optics. (B) Differential centrifugation analysis of peroxisomal targeting of the GFP-Pex8pΔAKL fusion in wild-type, Δpex7, and Δpex20 cells using the anti-Pex8p antibody.
