Figure 7.

Pex14p, but not Pex2p, is required for the peroxisomal entry of PpPex8p. Protease protection analysis of the P200 fraction (obtained directly from the PNS) of oleate-grown cells. Δpex14 (A) and Δpex2 (B) strains. The P200 fraction was incubated at room temperature with the indicated amounts of Proteinase K for 15 min, in the presence (+) or absence (-) of Triton X-100. Samples were subjected to SDS-PAGE and immunoblotting. (C) Cell lysates of oleate-grown wild-type, Δpex2, Δpex10, and Δpex12 cells were immunoblotted with the indicated antibodies.