Figure 4.

DdINCENP null cells have chromosome segregation defect. Time-lapse video microscopy of wild-type (top) and DdINCENP null cells (bottom) during mitosis, both of which expressed GFP-histone 2B. The micrographs were taken by fluorescence microscopy to show the movement of chromosomes (as indicated by GFP-histone 2B) from metaphase to anaphase in these two cells. The times are indicated in seconds. As shown, the wild-type cell segregated its chromosomes normally in a short time (86 s; see Video 5). However, the DdINCENP null cell displayed lagging chromosomes (arrows) during the segregation and stalled at anaphase (see Video 4). Other cells were arrested at metaphase for the entire observation period (see Video 3). Bar, 5 μm.