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Overexpression of ROCK(CAT) suppresses p21Cip1/Waf1 induction in PMA-treated K562 cell. K562 cells were transfected with pEF-neo or pEF-Myc-ROCK(CAT) by electroporation as described in Materials and Methods. After 24 h, cells were treated with PMA (50 nM) for the times indicated and harvested for Western blot analysis with antibodies against p21Cip1/Waf1, Myc, and TK.
