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. 2006 Feb;17(2):814–823. doi: 10.1091/mbc.E05-08-0729

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Copyright © 2006, The American Society for Cell Biology

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Figure 4. — Impaired recovery from nocodazole-induced mitotic arrest in Brd4+/- cells. (A) The percentage of apoptotic cells after nocodazole removal. Cells released from nocodazole treatment (100 ng/ml for 8 h) were stained for Annexin V and analyzed by flow cytometry. The values represent the average of three experiments. (B) DNA contents after nocodazole withdrawal. Cells released from nocodazole as mentioned above were stained with propidium iodide and analyzed by flow cytometry. (C) Clonogenic survival after nocodazole treatment. Brd4+/+ and +/- cells were treated with indicated concentrations of nocodazole for 8 h. Two hundred mitotic cells were replated in a well and allowed to grow in fresh media, and colonies were counted 7 d later. Untreated Brd4+/+ and +/- cells yielded comparable numbers of colonies. Values represent the average of three independent assays.