Table 2.
Plasmids used in this study
| Plasmid | Description | Origin |
|---|---|---|
| pRS305 | LEU2 | Sikorski and Hieter (1989) |
| pRS306 | URA3 | Sikorski and Hieter (1989) |
| pRS315 | CEN, ARS, LEU2 | Sikorski and Hieter (1989) |
| pRS316 | CEN, ARS, URA3 | Sikorski and Hieter (1989) |
| YEp351GAPII | 2μ, LEU2, TDH3 promoter and terminator | Abe et al. (2003) |
| YEp352GAPII | 2μ, URA3, TDH3 promoter and terminator | Abe et al. (2003) |
| pMO13 | GFP-HDEL, CEN, ARS, URA3 | Okamoto et al. (2006) |
| pMF600 | GAS1, pRS306 | This study |
| pMF605 | gas1*, pRS306 | This study |
| pMF607 | HA-GAS1, pRS305 | This study |
| pMF608 | mRFP-GAS1, pRS305 | This study |
| pMF615 | HA-gas1*, pRS305 | This study |
| pMF616 | HA-gas1*, pRS306 | This study |
| pMF617 | mRFP-gas1*, pRS305 | This study |
| pMF634 | BST1, YEp351GAPII | This study |
| pMF636 | BST1(S236A), YEp351GAPII | This study |
| pMF641 | BST1-FLAG, YEp351GAPII | This study |
| pMF642 | BST1(S236A)-FLAG, YEp351GAPII | This study |
| pMF643 | BST1-FLAG, YEp352GAPII | This study |
| pMF644 | BST1(S236A)-FLAG, YEp352GAPII | This study |
| pMF834 | FLAG-KAR2, YEp352GAPII | This study |
| pMF848 | CPY*HA, pRS316 | This study |
| pMF874 | SHG, pRS305 | This study |
| pMF876 | SHg*, pRS306 | This study |
| pMF880 | SEC18, pRS315 | This study |