Table 1.
Average length of actin filaments
Preparation | Average length (μm) | Range (μm) | n |
---|---|---|---|
TgACT1 in vitro | 0.105 ± 0.064 | 0.020-0.363 | 164 |
TgACT1 in vitro + phalloidin | 0.144 ± 0.102 | 0.030-0.705 | 157 |
TgACT1 in vitro + JAS | 0.241 ± 0.153 | 0.045-0.785 | 257 |
Rabbit actin in vitro | 2.020 ± 1.710 | 0.050-8.920 | 164 |
TgACT1 in vivo | 0.181 ± 0.082 | 0.072-0.420 | 76 |
TgACT1 in vivo + JAS | 0.199 ± 0.830 | 0.600-0.464 | 62 |
Average lengths of actin filaments in the preparations listed were determined by measurement from negatively stained EM negatives. All values for TgACT1 were performed on untagged protein.