Figure 3.

Effect of choline deprivation on the growth and viability of cho2opi3 cells cultured in liquid medium at 30°C. (A) Growth of cho2opi3 upon transfer of mid-log cells from synthetic complete lactate medium containing 1 mM choline to synthetic complete lactate medium with (□) or without 1 mM choline (▪. Growth rates were monitored by OD₆₀₀. (B) Viability of cho2opi3 cells after transfer of mid-log cells from lactate- (□) or glucose-based (▵) synthetic defined medium containing 1 mM choline to the corresponding medium with (open) or without 1 mM choline (solid). Averaged values from two independent experiments are shown with the error bars representing the variation. For experimental details, see Materials and Methods.