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. 2006 Jan;13(1):145–149. doi: 10.1128/CVI.13.1.145-149.2006

FIG. 1.

FIG. 1.

Results of mPCR/RLB hybridization for 27 reference strains. Lanes 1 to 27 represent the 27 reference strains, in the same order as in Table 1; the 20 labeled probes are in the same order as in Table 2. Duplicate rows for the same probe represent different probe concentrations (0.2 or 0.4 pM, respectively). Probe targets in rows from top to bottom are as follows: rows 1 and 2, GBS species specific; 3 and 4, blank; 5 to 8, Cα or Bca protein gene (protein gene profile code A [see Table 1, footnote a]); 9 to 12, blank; 13 to 16, alp2; 17 to 20, alp3; 21 to 24, blank; 25 to 28, alp4; 29 to 31, rib (protein gene profile code R); 32 to 35, epsilon/alp1/alp5; 36 and 37, Cβ or Bac protein gene (protein gene profile code B); 38 and 39, blank; 40 and 41, GBS species-specific gene. PCR product of strain no. 18 hybridized with GBS-specific probes but with none of the protein gene probes. However, on retesting, its PCR product hybridized with alp3 probes.