TABLE 1.
Conventional serotyping, molecular serotype identification based on PCR/sequencing, and mPCR/RLB hybridization protein gene profile for 27 GBS reference strainsa
| Strain no.b | Strain identification | CS | MS | mPCR/RLB pgp |
|---|---|---|---|---|
| 1 | O90 (Rockefeller University)c | Ia | Ia | Epsilon |
| 2 | H36B (ATCC 12401; NCTC 8187)c | Ib | Ib | AB |
| 3 | 18 RS 21 (NCTC 11079)c | II | II | R |
| 4 | M 781 (ATCC BAA-22)c | III | III | R |
| 5 | 3139c | IV | IV | Epsilon |
| 6 | CJB 111 (ATCC BAA-23)c | V | V | Alp3 |
| 7 | SS 1214c | VI | VI | Epsilon |
| 8 | 7271c | VII | VII | Alp3 |
| 9 | JM9 130013c | VIII | VIII | Alp3 |
| 10 | A909 (NCTC 11078)c | Ia | Ia | AB |
| 11 | BM110c | III | III | R |
| 12 | NZRM 908 (NCDC SS 615)c | Ia | Ia | Alp2 |
| 13 | NZRM 909 (NCDC SS 618)c | Ib | Ib | AB |
| 14 | NZRM 910 (NCDC SS 700)c | Ia | Ia | AB |
| 15 | NZRM 911 (NCDC SS 619)c | II | II | R |
| 16 | NZRM 912 (NCDC SS 620)c | III | III | Alp2 |
| 17 | NZRM 2832 (Prague 1/82; ATCC 49446)c | IV | IV | Epsilon |
| 18 | NZRM 2833 (Prague 10/84; ATCC 49447)c | V | V | Alp3 |
| 19 | NZRM 2834 (Prague 118754)c | VI | VI | AB |
| 20 | NZRM 2217 (Prague 25/60; ATCC 49448)d,e | NT | II | Alp4 |
| 21 | O90 (ATCC 12400)f | Ia | Ia | Alp2 |
| 22 | A909 (NCTC 11078)f | Ia | Ia | AB |
| 23 | 335 (NCTC 12906)f | Ia | Ia | Epsilon |
| 24 | 70339 (NCTC 12907)f | Ia | Ia | ARB |
| 25 | 65604 (3)f | III | III | R |
| 26 | 15626/81 (3)f | IV | IV | AB |
| 27 | Pattison (NCTC 9828)f | NT | II | Alp4 |
Abbreviations: CS, conventional serotype; MS, molecular serotype (serotype-specific PCR and/or partial cps sequencing; mPCR/RLB, reverse line blot assay, following multiplex PCR (all results were identical between individual PCR and mPCR/RLB); NT, nonserotypeable; A, Bca (Cα), R, Rib; B, Bac (Cβ); Epsilon, Epsilon/Alp1/Alp5; pgp, protein gene profile.
Strain numbers correspond to lane numbers in Fig. 1.
Eleven GBS reference strains from Lawrence Paoletti and Catherine Lachenauer, Channing Laboratory, Boston, Mass.
Nine GBS reference strains from Diana Martin, Streptococcus Reference Laboratory, at the Institute of Environmental Science and Research Limited, Wellington, New Zealand.
This isolate was the atypical reference strain Prague 25/60, which was nonserotypeable using capsular polysaccharide antisera but was assigned to molecular serotype II. It expresses a protein that is antigenically similar to R (and is used to generate R antiserum), encoded by the gene alp4 (12).
Seven GBS reference strains from Johan Maeland, Department of Microbiology, School of Medicine, Norwegian University of Science and Technology, Trondheim, Norway.