TABLE 2.

Oligonucleotide primers used in this study

Primera	Target	Tm (°C)b	GenBank accession no.	Sequencec
cfbSb	cfb	59.93	X72754	326 ATG ATG TAT CTA TCT GGA ACT CTA GTG 352
Sag59PSd	cfb	58.55	X72754	368 TTT TCA CCA GCT GTA TTA GAA GTA 391
Sag190PAd	cfb	59.74	X72754	522 GTT CCC TGA ACA TTA TCT TTG AT 500
cfbAb	cfb	59.74	X72754	585 CGC AAT GAA GTC TTT AAT TTT TC 563
bcaSb	bca	60.45	M97256	313 GCT TAC ATA GAT TTA TAT GAT GTA AAA TTA GG 344
bcaSp	bca	57.62	M97256	370 GTT TTA GAA CAA GGT TTT ACA GC 392
bcaAp	bca	58.60	M97256	579 CT TAT CCC TCA AGG TTG TTG 560
bcaAb	bca	58.91	M97256	637 CAG TAC GAC TTT CTT CCG TC 618
alp23Sb	alp2/alp3	59.89	AF208158	1254 CAT GGA AGT GAC AAT TAT GAA AG 1276
alp2Sp	alp2	59.24	AF208158	1366 CTT CCG CCA GAT AAA ATT AAG 1386
alp2Ap	alp2	58.25	AF208158	1576 CTG TTG ACT TAT CTG GAT AGG TC 1554
alp2Ab	alp2	60.81	AF208158	1603 CCA CTG TAA CTT TTA CAG GAA CTT C 1579
Alp23Sb	alp2/alp3	59.89	AF291065	1534 CAT GGA AGT GAC AAT TAT GAA AG 1556
alp3Sp	alp3	57.11	AF291065	1643 GTT CTT CCG CTT AAG GAT AG 1662
alp3Ap	alp3	60.17	AF291065	1711 CGG TGT TTC TCC TAC TTT GAC 1691
alp3Ab	alp3/rib	59.99	AF291065	1847 CTT TTG AAC CAT CTG GGT AAG 1827
ribSb	rib	63.88	U58333	219 AGA TAC TGT GTT TGC AGC TGA AGT AA 244
ribSp	rib	59.07	U58333	256 G CTG TTA CGT TAA ACA CAA ATA TG 279
ribAp	rib	57.92	U58333	448 C AAC AGT AGT CAA TTC AGA AGG 427
ribAb	rib	57.16	U58333	577 CTA TTT TAT CTC TCA AAG CTGAAG 554
alp4Sb	alp4	61.47	AJ488912	158 TGT TAG CAG CTG AAG TAG TTG AAG 181
alp4Sp	alp4	63.70	AJ488912	187 GCT GCA ACA TTA AAT ACT GCC AT 209
alp4Ap	alp4	60.62	AJ488912	325 TGT AAT AAA TAG CAG TGT ATC CCG 302
alp4Ab	alp4	60.05	AJ488912	350 GCA TAA ACT TTT GAA CCT TGT G 329
epsilonSb	epsilon	64.75	U33554	304 CTG TGT TTG CAG CTG AGG TG 323
epsilonSp	epsilon	58.30	U33554	452 CCC TTC TAA TTA TTC AGC AAA C 473
epsilonAp	epsilon	60.91	U33554	685 GGA TCA TTT GCA TTT TCA ATT AC 663
epsilonAb	epsilon	62.52	U33554	719 CA GTA CAT CTT TTC GAC TAT CAT CG 695
GBS1360Sb	bac	65.82	X59771	1337 AAG GCT ATG AGT GAG AGC TTG GAG 1360
GBS1716Sp	bac	58.32	X59771	1697 AAA AGT GAT TCG AAG ACG AC 1716
GBS1716Ap	bac	59.28	X59771	1735 AAG ATC ACT GAA GTC CAA CG 1716
GBS1937Ab	bac	64.80	X59771	1960 CTG CTC TGG TGT TTT AGG AAC TTG 1937

^aSuffixes: “b” indicates primers 5′ labeled with biotin; “p” indicates probes 5′ labeled with amine.

^bThe primer T_m values are those provided by the primer synthesizer (Sigma-Aldrich).

^cNumbers represent the numbered base positions at which primer sequences start and finish (numbering start points 1 refer to the start point 1 of the corresponding gene GenBank accession number).

^dThe species-specific primers have been previously published (9). All other primers were designed specifically for this study.