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. Author manuscript; available in PMC: 2006 Jan 31.
Published in final edited form as: J Am Chem Soc. 2005 Nov 30;127(47):16414–16415. doi: 10.1021/ja0562651

Figure 1.

Figure 1

Product distribution with 4-CH3-phenylalanine as substrate for the aromatic amino acid hydroxylases: 4-HOCH2-phenylalanine (dark gray), 3-CH3-4-HO-phenylalanine (light gray), and 3-HO-4-CH3-phenylalanine (white). Reactions were carried out and the products analyzed by HPLC as described in ref 6. Conditions: 25 mM sodium phosphate buffer, pH 7.0, 30μM ferrous ammonium sulfate, 1.2 mM 4-CH3-phenylalanine, 10 μM enzyme, and 150 μM 6-methyltetrahydropterin at 30 °C.