TABLE 2.
ART-deficient T cells efficiently upregulate activation antigens CD69 and CD25 upon T-cell receptor triggering even in the presence of added NADa
| Cells | NAD added | % Positive cells
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Unstimulated
|
Anti-CD3
|
Anti-CD3 + anti-CD28
|
Anti-CD3 + interleukin-2
|
||||||
| +/+ | −/− | +/+ | −/− | +/+ | −/− | +/+ | −/− | ||
| CD69+ | − | 3.5 | 5.5 | 84.9 | 82.1 | 78.1 | 80.1 | 88.6 | 79.8 |
| + | 8.1 | 7.0 | 36.7 | 58.9 | 32.6 | 58.5 | 38.5 | 63.1 | |
| CD25+ | − | 7.7 | 8.8 | 62.2 | 61.7 | 57.2 | 62.2 | 69.2 | 52.2 |
| + | 8.0 | 8.1 | 9.1 | 14.6 | 13.2 | 17.6 | 17.0 | 31.0 | |
a
T cells (5 × 105/ml of RPMI medium) were incubated for 24 h in the presence or absence of 50 μM NAD before staining as in Fig. 4. Numbers indicate percentages of marker-positive cells. +/+, wild-type mice; −/−, ART2-deficient mice.