TABLE 3.
Enhanced survival of ART2-deficient T cells in the presence of exogenous NADa
| NAD added | % Vital cells
|
|||||||
|---|---|---|---|---|---|---|---|---|
| Unstimulated
|
Anti-CD3
|
Anti-CD3 + anti-CD28
|
Anti-CD3 + interleukin-2
|
|||||
| +/+ | −/− | +/+ | −/− | +/+ | −/− | +/+ | −/− | |
| − | 65.8 | 73.1 | 60.4 | 72.1 | 25.4 | 47.2 | 18.9 | 20.4 |
| + | 23.9 | 56.6 | 27.5 | 68.6 | 11.7 | 46.9 | 8.9 | 30.9 |
a
Cells (5 × 105/ml of RPMI medium) were incubated for 24 h in the presence or absence of 50 μM NAD before staining as in Fig. 4. Dead cells were stained with propidium iodide. Numbers indicate percentages of vital cells. +/+, wild-type mice; −/−, ART2-deficient mice.