FIG. 7.

Human Gtpbp3 is a mitochondrial protein. 143B cells were transiently transfected with pEGFP-N1-GTPBP3. (A) Intracellular distribution of GTPBP3-GFP fusion protein was visualized by using the GFP antibody conjugated with Alexa Fluor 488. (B) The expression of the COX1 subunit in the same cell was assessed by the successive application of monoclonal antibody against human COX1 conjugated with Alexa Fluor 594. (C) A superimposition of panels A and B shows the complete overlap of the two staining patterns.