FIG. 4.

Effects of xCLOCK and xBMAL1 on PE, NE, and CE promoter activities in transiently transfected Cos 7 cells. The sequences of the different E-box-like elements shown are listed in the legend for Fig. 2A. The null-renilla vectors were cotransfected as internal controls. Sample values are means ± standard errors of determinations with four samples. All results were normalized to the relative luciferase activity in cells transfected with the PE-PCE-luc reporter gene only, whose activity was assigned a value of 1. ***, P < 0.001 (the value is significantly different from that for the respective control [reporter only]) by analysis of variance by the Tukey-Kramer multiple-comparison test. These data are representative of results from three independent experiments.