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General requirement of Spt3p but not Spt8p in recruitment of TBP to SAGA-dependent promoters. Wild-type (WT), spt3Δ, and spt8Δ deletion mutants were grown as described in the legend to Fig. 2 prior to formaldehyde treatment. Primer pairs located in the core promoters of the GAL1, ADH1, VTC3, BDF2, and PHO84 genes were used for PCR analysis of the input and immunoprecipitated (IP) DNA samples.
