FIG. 5.

Differential requirement of SAGA TAFs for recruitment of TBP to SAGA-dependent promoters. Yeast strains harboring temperature-sensitive mutations in TAF9, TAF6, and TAF12 were first grown at 23°C to an OD₆₀₀ of 0.8 and then transferred to 37°C for 1 h prior to treatment with formaldehyde. Primer pairs located in the core promoters of the GAL1, ADH1, VTC3, BDF2, and PHO84 genes were used for PCR analysis of the input and immunoprecipitated (IP) DNA samples.