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Override of HIRA-induced S-phase arrest by SLBP requires binding of SLBP to 3′ UTR of histone mRNA. (a) U2OS cells were transiently transfected with pcDNA3 or pcDNA3 HA-HIRA WT in the absence or presence of pcDNA3 HA-SLBP WT, pcDNA3 HA-SLBP RR/KK, or pcDNA3 HA-SLBP QKQ (0.2 or 1 μg [triangles]) together with pCMV-CD19. Cells were harvested, and the cell cycle distribution was determined by FACS. (b) Lysates were Western blotted with antibodies to HA.
