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. 2002 Nov;22(21):7645–7657. doi: 10.1128/MCB.22.21.7645-7657.2002

FIG. 2.

FIG. 2.

Defective SCF-induced protease expression in Rac2-deficient mast cells. (A) Steady-state levels of MMCP-4 and MMCP-7 mRNAs in response to SCF and IL-3. Wild-type (WT) and Rac2−/− C57BL/6 BMMCs maintained in IL-3-containing medium were stimulated with 10 ng of SCF per ml, and 10 μg of each total RNA sample from 6-week-old C57BL/6 BMMCs was used per lane. Levels of mouse β-actin mRNA were used as a loading control. (B) MMCP-7 mRNA levels in wild-type C57BL/6 and 129/Sv BMMCs. Cells cultured in the presence of IL-3 for 2 weeks were stimulated with SCF for 4 h. We used 10 μg of each total RNA sample per lane, and ethidium bromide-stained 28S and 18S RNAs were used as a loading control. (C) MMCP-7 mRNA levels in wild-type (WT) and Rac2−/− 129/Sv BMMCs. 129/Sv BMMCs from 2- or 6-week-old cultures were stimulated with 10 ng of SCF per ml for 4 h. We used 5 μg of each total RNA sample in lanes 1 to 4 and 10 μg of each total RNA sample in lanes 5 to 8. (D) Restoration of SCF-induced MMCP-7 mRNA levels in Rac2−/− C57BL/6 BMMCs. Rac2−/− BMMCs were transduced with a retroviral vector, MIEG3-FR2, expressing wild-type Rac2 [designated Rac2−/− (Rac2)]. Cells from 2- or 6-week-old cultures were stimulated with 10 ng of SCF per ml for 4 h. We used 5 μg of total RNA sample per lane. All results shown are representative of three experiments.