FIG. 7.

Phosphatidylinositol 3-kinase and JNK activities are required for SCF-induced MMCP-7 expression in mast cells. Wild-type C57BL/6 BMMCs were stimulated with 10 ng of SCF per ml with or without the kinase inhibitors Ly294002 (20 μM) for phosphatidylinositol 3-kinase, PD98059 (50 μM) for MEK, 420116 (1 μM) for JNKs, SN50 (20 μM) for NF-κB, and SB203580 (40 μM) for p38 MAPK, for 4 h. We used 10 μg of total RNA for Northern blot analysis with a gene-specific probe for MMCP-7. The ethidium bromide-stained 28S and 18S RNAs were used as a loading control. The hybridization intensity is represented in arbitrary units in the bottom panel. Similar results were seen in two other experiments.