FIGURE 1. Fluorescent micrographs of hepatocytes after overnight culture following cryopreservation. Cells were cryopreserved in (B) HTS + 10% DMSO (HTS-cryo) or (C) culture media + 10% DMSO (Media-cryo), cultured overnight on collagen coated tissue culture ware and compared with (A) nonfrozen controls. Samples were stained with Syto13/ethidium bromide to distinguish living populations (green cells) and dead populations (red cells).