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Vectors used for construction of plasmids. Vectors pCMV-mcs, pXK909, and pCMV-Gal4 were used to express variants of ICP4 in transfected cells. Vectors pXK990 and pXK992 were used to construct genes for insertion into the ICP4 locus of the HSV genome by homologous recombination. Genes were inserted into the multiple cloning site (mcs). The numbers below the ICP4 promoter indicate the base position relative to the transcription initiation site.
