TABLE 3.

Activities of ICP4 variants in HSV-1-infected cells^a

Transfected ICP4	Cells infected with:
	HSV-1072		HSV-976
	Fold activationc	% of wild type activation	PFU/dish	Fold complementationd
Noneb	1.0	0.0	5.5 × 103	1.0
Wild type	77	100	5.5 × 105	100
ΔTAD1	59	76	2.2 × 105	40
Mut1	20	24	9.6 × 104	18
ΔTAD1 + Mut1	5.2	5.5	2.4 × 104	4.4

^aVero cells were transfected with a plasmid (100 ng) encoding the indicated ICP4 variant and infected with the indicated virus 24 h posttransfection.

^bCells were transfected with a control plasmid.

^cFold activation of the TK-luciferase gene in HSV-1072 4 h postinfection.

^dRatio of virus yields from cells transfected with the indicated ICP4-encoding plasmid versus a control plasmid.